Tester Site

Please Note This website is a tester for page layout and data visualization for the Lipp Lab COVID Wastewater monitoring project. The data on this page are not peer-reviewed or finalized for public release and thus should not be interpreted as such. If you are interesed in viewing the final data from this project please visit this website.

Data Trends

The total viral load of SARS‑CoV‑2 in the sewershed was estimated using data from our weekly wastewater monitoring. Sewage samples are collected weekly from two of the three water reclamation facilities that serve Athens Clarke County (WRF A and WRF B). Samples are tested to detect and measure SARS‑CoV‑222, the virus that causes COVID‑19.

The total viral load of SARS‑CoV‑2 is estimated by adjusting for the total wastewater volume collected by each water reclamation facility. The estimate is expressed as a sum of the total number of gene targets detected between the two facilities.

The trendlines below reflect the estimated total viral load of SARS‑CoV‑2 in the sewershed. The viral load is estimated by the N1 (green) and N2 (orange) gene targets. Data trends were calculated using locally estimated scatterplot smoothing (LOESS, span = 0.6). The error associated with these estimates (the 95% confidence interval) is displayed.

Dashed lines indicate significant events that may affect local public health. On June 24, 2020 Georgia continues broad reopening, including bars. July 9, 2020 Athens-Clarke County implements mask mandate. On August 20, 2020 classes begin at the University of Georgia.

We predict that changes in the concentration of SARS‑CoV‑22 in wastewater will reflect the community trends of circulating COVID‑19 infections. The daily number of newly reported COVID‑19 cases in Athens-Clarke County (purple) appear alongside the 7-day moving average of new cases (yellow).

Weekly Data Update

Updated every Thursday
Please Note: We strive to get results for each week’s sample updated by 5:00 pm on Thursday however, to ensure quality of data occasional delays in the update may occur. We will post updated data as soon as we are able.

Oct 20

SARS‑CoV‑2 concentrations in Athens Clarke County wastewater showed a consistent upward trend this week. WRF A continued its increase from the prior week with concentrations reaching as high as 1 million copies per L. Concentrations at WRF B increased from the below the limit of detection to >300,000 copies per L. The continued upward trends may suggest changes in viral shedding within the community.

Reported cases in Athens Clarke County have been stable over the week, with a 7-day running average of 20-30 cases per day.

Oct 13

Trends in SARS CoV 2 concentrations in Athens wastewater were split this week between the two sampled plants. During the collection period (Oct 11 – 12), viral RNA concentrations at WRF B declined to at or below the limit of detection. However, levels at WRF A increased to >500,000 copies per L, after declining to near the limit of detection the prior week. It is too early to know if the increased SARS CoV 2 signal at WRF A is indicative of possible uptick in transmission or due to inherent variability in sample collection. If there is increased transmission, we would expect to see the trend in wastewater continue next week as well as an increase in reported cases.

Reported cases in Athens Clarke County have remained stable for the last 2 weeks (7-day running average between 21 and 24 new cases per day).

Oct 6

Levels of SARS-CoV-2 continues to decrease in wastewater samples collected from Athens-Clarke County. These results are consistent with the decrease in the number of newly-reported cases over the past two weeks.

It is important to note that the levels of SARS-CoV-2 virus have decreased to or at the limit of detection for our current method. Our theoretical limit of detection is approximately 357,000 copies/L. Below this concentration, viruses may be not be detected or quantified with accuracy.

Sept 29

Overall SARS-CoV-2 concentrations continued to decline in sewage reaching Athens’s WRFs, in line with decreasing reported cases in Athens Clarke County. The decline was especially evident at WRF A, where levels were at or below the limit of detection for our methods. However, concentrations at WRF B were virtually unchanged from last week.

With our current process, we cannot accurately detect virus concentrations below about 3,000 copies per L. Therefore, we cannot assume that a sample that is ‘below the limit of detection’ is negative for SARS-CoV-2, rather it may be at some level that we cannot accurately determine with our current method. Our lab is evaluating different detection options that may improve detection during times with lower levels of circulating virus.

Sept 22

Concentrations of SARS‑CoV‑2 declined this week in wastewater collected from both WRFs. Average levels remain above 100,000 copies/L. This decline is consistent with declines in reported cases in Athens-Clarke County.

Sept 15

The September 15 update is experiencing a delay and will be posted by Friday Sept 18. We apologize for any inconvenience

Sept 8

This week, levels of SARS‑CoV‑2 for both targets at both plants continued to exceed 1 million copies of virus per liter of sewage. Average values were similar to concentrations from the prior week (September 1, 2020) with virtually no change in the average levels at WRF B and a 6% reduction at WRF A.

An important note about sample collection this week was that it occurred over the Labor Day holiday and may explain the slight reduction in viral concentration at the WRF A plant. Samples were collected between ~6:30 am Monday through 6:30 am Tuesday. The volume of wastewater flowing into WRF A decreased from 5.73 million gallons on September 1, 2020 to 4.96 million gallons on September 8, 2020. The collection area for WRF A includes the UGA main campus and Downtown Athens. We suspect that this week’s data point reflects the real-time effect of population movement on viral levels in wastewater. In other words, a closed campus and likely large number of people traveling reduces the input. Samples collected on a particular day reflect the inputs of that day and not necessarily the entire week.

Our trend analysis suggests a continued elevation in total viral load (see data trends tab).

Daily reported cases in Clarke County continue to rise, now reaching a 7-day moving average of 126.1 new cases per day.

Sept 1

This week continues the trend of the past month with SARS‑CoV‑2 concentrations increasing in wastewater. Levels increased to their highest point since the beginning of data collection with average concentrations for both plants and both targets exceeding 3 million copies of virus per liter of sewage, approximately 10-fold higher than the prior week. Daily reported cases in Clarke County also continued to rise reaching a 7-day moving average of >74 cases per day.

Aug 25

SARS-CoV-2 concentrations in Athens-Clarke County wastewater increased slightly in this week’s samples (reaching >900,000 copies of the virus per liter of sewage). This moderate increase continues the upward trend observed over the prior three weeks. Reported cases have remained steady through the week (8/18 - 8/25/2020) averaging ~30 new cases per day.

Aug 18

On August 18, 2020, the concentration of SARS‑CoV‑2 increased to over 100,000 copies per liter for both gene targets. This uptick is somewhat reflected in the daily reported cases for Athens-Clarke county and may indicate further increases in the coming weeks.

Furthermore, this week marks the return of approximately 30,000 undergraduate students to the UGA main campus for the start of the fall semester.

Prior to Aug 18, 2020

Weekly analyses of SARS‑CoV‑2 in sewage began June 16, 2020, at which time concentrations were below the current method limit of detection and new cases were reported at <10 per day. Reported cases began to increase to >20 per day in late June and SARS‑CoV‑2 concentration increased to up to 10 million viral copies per liter. Sewage concentrations were steady through mid-July inline with newly reported cases averaging 35 per day. Beginning July 21, 2020 SARS‑CoV‑2 concentrations in sewage began to decline through early August, while concurrent case report remained high. We suspect that this decline possibly preceded the decline in cases noted in late August, which may be due to lags in testing results and case reporting.

Since August 4, 2020 SARS‑CoV‑2 concentration has steadily increased for both the N1 and N2 gene targets. This suggests that sewage concentrations may be a leading indicator for new cases in the community, but more data collection and monitoring of delays in testing reports will be needed to confirm this trend.

All Data

Sewage samples are collected weekly from water reclamation facilities that serve Athens Clarke County (WRF A and WRF B). Samples are tested to detect and measure SARS‑CoV‑2, the virus that causes COVID‑19.

We measure the virus by detecting two genes specific to SARS‑CoV‑2, the N1 and N2 nucleocapsid genes, using RT-qPCR. The N1 and N2 gene targets are measured and reported as a concentration, in number of gene copies per liter of wastewater. The theoretical Limit of Detection (LOD) is noted.

We predict that changes in the concentration of SARS‑CoV‑2 in wastewater will reflect the community trends of circulating COVID‑19 infections. The daily number of newly reported COVID‑19 cases in Athens-Clarke County (purple) appear alongside the 7-day moving average of new cases (yellow). Changes in SARS‑CoV‑2 in wastewater may precede changes in reported cases because of lags in clinical testing.

Individual Plants

WRF A

WRF B

WRF C

Research Overview

Purpose

Wastewater surveillance is an emerging tool for monitoring disease outbreaks. Coupled with clinical surveillance, wastewater surveillance can be used to estimate the level of viruses circulating in local population.

To better understand the outbreak of the novel Coronavirus disease-19 (COVID‑19) on a local level, we are monitoring wastewater samples from water reclamation facilities in Athens, GA. Wastewater samples are collected weekly and tested to detect the disease-causing virus, SARS‑CoV‑2.

Each sample is a composite of the wastewater received by the water reclamation facility in a single day. By examining these samples, we are capturing a snapshot into the health of the entire community that contributes to the sewershed. This wastewater-based research provides a new approach to epidemiology; sewage surveillance may be used to predict the total burden of disease at a community-scale, without extensive clinical sampling.

We are sharing here some of the preliminary results of our wastewater monitoring program. Our objective is to critically examine the use of SARS‑CoV‑2 wastewater-based epidemiology for the detection and surveillance of SARS‑CoV‑2 in our local community.

We are developing and evaluating our methods in real-time. We will share with you our weekly results and newest developments. This data is intended to complement clinical-based COVID‑19 surveillance data, that will together, inform public health decisions.

To learn more about national surviellence efforts, using wastewater-based epidemiology, please visit the CDC’s National Wastewater Surveillance System (NWSS).

Methods

Since May 2020, sewage samples have been collected weekly from the water reclamation facilities (WRFs) that serve Athens-Clarke County. Samples are collected from a 24-hour composite of the influent wastewater received by each WRF.

Samples are processed by the Lipp Laboratory at the University of Georgia to detect and measure the abundance of SARS‑CoV‑2 virus in wastewater.

Viral RNA, the genetic material of the SARS‑CoV‑2 virus, is extracted from each sample using the QIAmp Viral RNA Mini Kit.

The viral RNA is then measured using Real-Time RT-PCR Assays, following the CDC’s test protocol for the N1 and N2 SARS‑CoV‑2 nucleocapsid genes.

The N1 and N2 gene targets are measured and reported as a concentration, by the number of gene copies measured per liter of wastewater.

Reported case data for COVID-19 has been collected from Georgia’s Department of Public Health and summarized by Mark Ebell, MD, MS here.

A more detailed explanation of the research methods can be found here.

About

This weekly monitoring is conducted in partnership between Athens-Clarke County Utilities, the Lipp Laboratory, and the College of Public Health at the University of Georgia.

Team

Dr. Erin Lipp

Megan Lott, PhD Student

William Norfolk, PhD Student

Megan Robertson, PhD Student

For more information about our team and other ongoing research please visit the Lipp Laboratory website.